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If building v2 sample sheets for instruments other than the ones listed, affirm the final sample sheet has the i5 index in the right orientation as explained previously mentioned.
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When demultiplexing, the ultimate sample sheet furnished to your FASTQ era software program needs to contain the i5 index in the right orientation for that platform. However, most Illumina sample sheet technology computer software handles this immediately.
This allows large figures of libraries for being pooled and sequenced simultaneously all through one run. Compared to other indexing procedures, special twin indexing mitigates index hopping by filtering hopped reads.
The software program routinely makes the reverse enhance as required for Examination. In the event the sequence is reverse complemented, this can only be shown in the SampleSheetUsed.csv file.
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When examining index sets for colour equilibrium, the sequence observed by the instrument needs to be here assessed, regardless of the orientation useful for the sample sheet. To the i7 index, usually asses shade balance utilizing the "i7 bases for sample sheet" column orientation.
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